Phosphodiester hydrolysis computed for cluster models of enzymatic active sites

Enhancing Enzymatic Hydrolysis of Cellulose by Ultrasonic Pretreatment

Slurries of rice-straw cellulose (obtained by delignification and removal of hemicelluloses from the powdered raw material) were subjected to ultrasonic waves at different intensities for various times (constant temperature). Susceptibility of the samples to cellulose-hydrolysis increased initially with pretreatment time, reaching a maximum or a constant level thereafter. Maximum glucose yi...

Mechanistic Models on Enzymatic Hydrolysis and Anaerobic Digestion

Alkali metals (Li, Na, and K) in methyl phosphodiester hydrolysis.

The phosphodiester linkage central to biological systems has been modeled by methyl phosphodiester (MPDE) in various theoretical and experimental studies. Under physiological conditions, hydrolysis of the phosphodiester is negligible, however this process can be catalyzed in the presence of metal ions. To understand the role of alkali metals in MPDE hydrolysis and, in particular, how it influen...

Transcript assisted phosphodiester bond hydrolysis by eukaryotic RNA polymerase II

Hydrolysis of the phosphodiester bonds of the transcript by bacterial RNA polymerase is assisted by 3′NMP of the RNA. Here we provide evidence that this mechanism is also involved in RNA cleavage by eukaryotic RNA polymerase II, suggesting that transcript assisted hydrolysis has emerged before divergence of bacteria and archaea/eukaryotes. Multisubunit RNA polymerase (RNAP), the enzyme accompli...

Effects of Condition Enzymatic Hydrolysis on Degrees of Hydrolysis and Antioxidant Activity of Head Protein of Bighead (Aristichthys nobilis)

Background and Objectives: Enzymatic hydrolysis is an effective practical technology that recovers valuable proteins from far-less farmed fish without losing their nutritional characteristics. Enzymatic hydrolysis of protein sources such as aquatic animals is a type of protein recycling. In the present study, effects of temperature, time and concentration of papain enzyme on hydrolysis degree a...